Abstract
Objective
We investigated the possible association of intracellular cytokine profiles in peripheral
mononuclear cells in whole blood from patients with carotid atherosclerosis in whom
follow-up after carotid endarterectomy (CEA) showed the onset or progression of contralateral
disease.
Methods and results
Intracellular expression of tumor necrosis factor (TNF)-α, interferon (IFN)-γ, interleukin
(IL)-1β, IL-6, IL-8, IL-4 and IL-10 was determined in 43 patients with carotid atherosclerosis,
at baseline and at 1, 3, 6 and 12 months after CEA, and in 16 healthy subjects. When
follow-up ended patients were divided into two groups, those with cured or stable
disease (no onset of disease or unchanged stenosis in the contralateral vessel) and
those with progressive disease (onset of disease or increased stenosis in the contralateral
vessel). In patients with cured or stable disease, cytokine-positive cells significantly
decreased or remained unchanged (IL-8 and IL-10) during follow-up, whereas in patients
with progressive disease they increased or remained unchanged (TNF-α). Multivariate
ANOVA confirmed that the trend of cytokine expression between baseline and 12 months
differed significantly in the two groups.
Conclusions
Intracellular expression of TNF-α, IFN-γ, IL-1β, IL-6, IL-8, IL-4 and IL-10 in peripheral
mononuclear cells is associated with the outcome of contralateral disease after CEA.
Keywords
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Article info
Publication history
Published online: May 09, 2006
Accepted:
March 22,
2006
Received in revised form:
March 21,
2006
Received:
January 11,
2006
Identification
Copyright
© 2006 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.
