Abstract
Apo E plays an important role in chylomicron and VLDL remnant processing, uptake or
conversion to LDL. The type of lipoprotein that isolates in the LDL density of E2/2
subjects was investigated and the effect of the apo E isoforms on LDL mass was determined
in all genotypes in a large group of Type 1 diabetics. Analysis of the LDL composition
of E2/2 homozygotes (n = 6) compared to subjects with the common E3/3 isoform (n = 6) demonstrated an enrichment in apo E, unesterified cholesterol, phospholipid and
triglyceride relative to apo B in E2/2 subjects, more typical of a dense IDL remnant
than of LDL. Although diabetics were studied, these findings are considered to reflect
those of the general population. Comparison of the lipoprotein distribution of homozygous
and heterozygous subjects revealed that, as genotype changed from E4/4 (n = 22) to E3/4 (n = 262), E3/3 (n = 710) = E2/4 (n = 30), E2/3 (n = 151), E2/2 (n = 6), LDL cholesterol decreased significantly in a stepwise manner. The decrease was
not in a specific subgroup of LDL. In conclusion, for E2/2 subjects, lipoproteins
isolated in the LDL density range appear to be composed mainly of dense IDL remnants
and some Lp(a). The apo E isoform also has a significant effect on LDL concentration
in both homozygotes and heterozygotes.
Abbreviations:
Apo E (apolipoprotein E), DGUC (density gradient ultracentrifugation)Keywords
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Article info
Publication history
Published online: June 01, 2006
Accepted:
April 7,
2006
Received in revised form:
March 22,
2006
Received:
September 28,
2005
Identification
Copyright
© 2006 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.
