Advertisement

Stent implantation in coronary porcine arteries is associated with early activation of TNFα and TNFα receptor II expression

      Abstract

      Inflammation present in restenosis after angioplasty is associated with production of cytokines such as tumor necrosis factor (TNFα). However, limited data exist on the possible increase in TNFα and TNFα receptor expression induced during the chronic phase after stenting. To this end, swine underwent balloon denudation (PTCA) and stent implantation in coronary arteries. At day 1, 7 or 28 post-procedure, sections from injured and reference vessel segments were evaluated for extent of pathology and expression of TNFα and TNFα receptors (RI and RII). Restenosis assessed at days 7 and 28 showed, respectively, two- and six-fold more neointimal (NI) area in stented than in PTCA segments. Unlike reference segments, TNFα-positive cells were detected in both the media and the NI of injured segments, with a significant increase over the 28-day time frame. Stenting was associated with an eight-fold enhancement in TNFα expression over PTCA. TNFα expression and NI area tended to correlate in injured segments. Furthermore, the pattern of expression of TNFα-RII, but not TNFα-RI, resembled that of TNFα itself. These results implicate TNFα and TNFα-RII as important actors in both the acute and the chronic phases of inflammation following stent implantation.

      Keywords

      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribe:

      Subscribe to Atherosclerosis
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect

      References

        • Ellis S.G.
        • Savage M.
        • Fishman D.
        • et al.
        Restenosis after placement of Palmaz-Shatz stents in native coronary arteries: initial results of a multicenter experience.
        Circulation. 1992; 86: 1836-1844
        • McFadden E.P.
        • Stabile E.
        • Regar E.
        • et al.
        Late thrombosis in drug-eluting coronary stents after discontinuation of anti-platelet therapy.
        Lancet. 2004; 364: 1519-1521
        • Ong A.T.
        • Serruys P.W.
        Technology insight: an overview of research in drug-eluting stents.
        Nat Clin Pract Cardiovasc Med. 2005; 12: 647-658
        • Pai J.K.
        • Pischon T.
        • Ma J.
        • et al.
        Inflammatory markers and the risk of coronary heart disease in men and women.
        N Engl J Med. 2004; 351: 2599-2610
        • Mazzone A.
        • Cusa C.
        • Mazzucchelli I.
        • et al.
        Increased production of inflammatory cytokines in patients with silent myocardial ischemia.
        J Am Coll Cardiol. 2001; 38: 1895-1901
        • Tashiro H.
        • Shimokawa H.
        • Sadamatsu K.
        • et al.
        Role of cytokines in the pathogenesis of restenosis after percutaneous transluminal coronary angioplasty.
        Coron Artery Dis. 2001; 12: 107-113
        • Krishnaswamy G.
        • Kelley J.
        • Yerra L.
        • et al.
        Human endothelium as a source of multifunctional cytokines: molecular regulation and possible role in human disease.
        J Interferon Cytokine Res. 1999; 19: 91-104
        • Karas S.P.
        • Gravanis M.B.
        • Santoian E.C.
        • et al.
        Coronary intimal proliferation after balloon injury and stenting in swine: an animal model of restenosis.
        J Am Coll Cardiol. 1992; 20: 467-474
        • Chandrasekar B.
        • Nattel S.
        • Tanguay J.F.
        Coronary artery endothelial protection after local delivery of 17beta-estradiol during balloon angioplasty in a porcine model: a potential new pharmacologic approach to improve endothelial function.
        J Am Coll Cardiol. 2001; 38: 1570-1576
        • Murphey E.D.
        • Traber D.L.
        Pretreatment with tumor necrosis factor alpha attenuates arterial hypotension and mortality induced by endotoxin in pigs.
        Crit Care Med. 2000; 28: 2015-2021
        • Schwartz R.S.
        • Huber K.C.
        • Murphy J.G.
        • et al.
        Restenosis and the proportional neointimal response to coronary artery injury: results in a porcine model.
        Am Coll Cardiol. 1992; 19: 267-274
        • Tanguay J.F.
        • Hammoud T.
        • Geoffroy P.
        • et al.
        Chronic platelet and neutrophils adhesion: a causal role for neointimal hyperplasia in in-stent restenosis.
        J Endovasc Ther. 2003; 10: 968-977
        • Clausell N.
        • de Lima V.C.
        • Molossi S.
        • et al.
        Expression of tumour necrosis factor alpha and accumulation of fibronectin in coronary artery restenotic lesions retrieved by atherectomy.
        Br Heart J. 1995; 73: 534-539
        • Tanaka H.
        • Sukhova G.
        • Schwartz D.
        • Libby P.
        Proliferating arterial smooth muscle cells after balloon injury express TNF-alpha but not interleukin-1 or basic fibroblast growth factor.
        Arterioscler Thromb Vasc Biol. 1996; 16: 12-18
        • Aggarwal B.B.
        Signalling pathways of the TNF superfamily: a double-edged sword.
        Nat Rev Immunol. 2003; 3: 745-756
        • Idriss H.T.
        • Naismith J.H.
        TNF alpha and the TNF receptor superfamily: structure–function relationship(s).
        Microsc Res Tech. 2000; 50: 184-195
        • Wang Z.
        • Rao P.J.
        • Castresana M.R.
        • et al.
        TNF-alpha induces proliferation or apoptosis in human saphenous vein smooth muscle cells depending on phenotype.
        Am J Physiol. 2005; 288: H293-H301
        • Goetze S.
        • Xi X.P.
        • Kawano Y.
        • et al.
        TNF-alpha-induced migration of vascular smooth muscle cells is MAPK dependent.
        Hypertension. 1999; 33: 183-189
        • McCarthy N.J.
        • Bennett M.
        The regulation of vascular smooth muscle cell apoptosis.
        Cardiovasc Res. 2000; 45: 747-755
        • Fotin-Mleczek M.
        • Henkler F.
        • Samel D.
        • et al.
        Apoptotic crosstalk of TNF receptors: TNF-R2-induces depletion of TRAF2 and IAP proteins and accelerates TNF-R1-dependent activation of caspase-8.
        J Cell Sci. 2002; 115: 2757-2770
        • Zhou Z.
        • Lauer M.A.
        • Wang K.
        • et al.
        Effect of anti-tumor necrosis factor-alpha polyclonal antibody on restenosis after balloon angioplasty in a rabbit atherosclerotic model.
        Atherosclerosis. 2002; 161: 153-159
        • Park S.J.
        • Kim H.S.
        • Yang H.M.
        • et al.
        Thalidomide as a potent inhibitor of neointimal hyperplasia after balloon injury in rat carotid artery.
        Arterioscler Thromb Vasc Biol. 2004; 24: 885-891
        • Carvalho-Tavares J.
        • Hickey M.J.
        • Hutchison J.
        • et al.
        A role for platelets and endothelial selectins in tumor necrosis factor-alpha-induced leukocyte recruitment in the brain microvasculature.
        Circ Res. 2000; 87: 1141-1148
        • Wang K.
        • Zhou Z.
        • Zhou X.
        • et al.
        Prevention of intimal hyperplasia with recombinant soluble P-selectin glycoprotein ligand-immunoglobulin in the porcine coronary artery balloon injury model.
        J Am Coll Cardiol. 2001; 38: 577-582
        • Tanguay J.F.
        • Geoffroy P.
        • Sirois M.G.
        • et al.
        Prevention of in-stent restenosis via reduction of thrombo-inflammatory reactions with recombinant P-selectin glycoprotein ligand-1.
        Thromb Haemost. 2004; 91: 1186-1193
        • Clausell N.
        • Molossi S.
        • Sett S.
        • et al.
        In vivo blockade of tumor necrosis factor-alpha in cholesterol-fed rabbits after cardiac transplant inhibits acute coronary artery neointimal formation.
        Circulation. 1994; 89: 2768-2779
        • Rectenwald J.E.
        • Moldawer L.L.
        • Huber T.S.
        • et al.
        Direct evidence for cytokine involvement in neointimal hyperplasia.
        Circulation. 2000; 102: 1697-1702
        • Grewe P.H.
        • Deneke T.
        • Machraoui A.
        • et al.
        Acute and chronic tissue response to coronary stent implantation: pathologic findings in human specimen.
        J Am Coll Cardiol. 2000; 35: 157-163
        • Feldman L.J.
        • Aguirre L.
        • Ziol M.
        • et al.
        Interleukin-10 inhibits intimal hyperplasia after angioplasty or stent implantation in hypercholesterolemic rabbits.
        Circulation. 2000; 101: 908-916
        • Javed Q.
        • Swanson N.
        • Vohra H.
        • et al.
        Tumor necrosis factor-alpha antibody eluting stents reduce vascular smooth muscle cell proliferation in saphenous vein organ culture.
        Exp Mol Pathol. 2002; 73: 104-111