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Influences of the phosphatidylcholine transfer protein gene variants on the LDL peak particle size

      Abstract

      Background

      The small, dense LDL phenotype is associated with an increased cardiovascular disease risk. A genome-wide scan performed on 236 nuclear families of the Quebec Family Study (QFS) revealed a quantitative trait locus (QTL) affecting LDL peak particle size (LDL-PPD) and density on the 17q21 region. This region contains the phosphatidylcholine transfer protein gene (PCTP). In the liver, phosphatidylcholine transfer protein binds specifically phosphatidylcholine suggesting a role for this protein in the formation of HDL and possibly VLDL phospholipid membranes.

      Objectives

      To test the association between two coding polymorphisms (c.29A>C (Glu10Ala) and c.188G>A (Cys63Tyr)) in PCTP gene and the LDL-PPD.

      Methods

      LDL-PPD was measured by non-denaturating 2–16% polyacrylamide gradient gel electrophoresis on 623 QFS subjects.

      Results

      After adjustment for age and sex, carriers of the c.29C allele showed larger LDL-PPD than A/A homozygotes (p < 0.05). These results remained significant when LDL-PPD was further adjusted for the effects of BMI and triglyceride levels (p < 0.04). We also observed a three-fold lower risk of having the small (LDL-PPD <256 Å), dense LDL phenotype in subjects carrying the c.29C allele, when compared to A/A homozygotes (OR = 0.35 (95% CI: 0.14–0.91; p = 0.03)).

      Conclusion

      PCTP gene variants are associated with LDL-PPD.

      Abbreviations:

      APOH (apolipoprotein H gene), CHD (coronary heart disease), GGE (gradient gel electrophoresis), HDL (high-density lipoprotein), HDL-C (high-density lipoprotein cholesterol), HDL2-C (high-density lipoprotein 2 cholesterol), HDL3-C (high-density lipoprotein 3 cholesterol), LDL (low-density lipoprotein), LDL-C (low-density lipoprotein cholesterol), LDL-PPD (low-density lipoprotein peak particle diameter), PCTP (phosphatidylcholine transfer protein gene), QFS (Quebec Family Study), QTL (quantitative trait locus), SNP (single nucleotide polymorphism), VLDL (very-low density lipoprotein)

      Keywords

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