Advertisement

Angiotensin II induces CD62L shedding in human neutrophils

  • Author Footnotes
    1 These authors contributed equally to this work.
    Antonio Vega
    Footnotes
    1 These authors contributed equally to this work.
    Affiliations
    Servicio Regional de Inmunología y Alergia, Hospital Universitario Virgen Macarena, Sevilla, Spain
    Search for articles by this author
  • Author Footnotes
    1 These authors contributed equally to this work.
    Rajaa El Bekay
    Footnotes
    1 These authors contributed equally to this work.
    Affiliations
    CIBER Fisiopatología Obesidad y Nutrición (CB06/03), Instituto de Salud Carlos III, Málaga 29009, Spain

    Laboratorio de Investigación Biomédica, Hospital Clínico Virgen de la Victoria, Málaga, Spain
    Search for articles by this author
  • Pedro Chacón
    Affiliations
    Servicio Regional de Inmunología y Alergia, Hospital Universitario Virgen Macarena, Sevilla, Spain

    Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER), Sevilla, Spain
    Search for articles by this author
  • Inmaculada Ventura
    Affiliations
    Servicio Regional de Inmunología y Alergia, Hospital Universitario Virgen Macarena, Sevilla, Spain
    Search for articles by this author
  • Javier Monteseirín
    Correspondence
    Corresponding author at: Asunción 27, 41011 Sevilla, Spain. Tel.: +34 954282075.
    Affiliations
    Servicio Regional de Inmunología y Alergia, Hospital Universitario Virgen Macarena, Sevilla, Spain
    Search for articles by this author
  • Author Footnotes
    1 These authors contributed equally to this work.

      Abstract

      Studies indicate that both alterations in leukocyte and endothelial cell adhesion molecules and the renin angiotensin system are involved in the pathogenesis of atherosclerosis processes in human hypertension. The present work was undertaken to investigate whether angiotensin II (Ang II) regulates the expression of CD62L on human neutrophils. Human neutrophils were stimulated with Ang II in the presence of various AT1-receptor antagonists and protein kinase inhibitors, and CD62L cell surface expression was detected by flow cytometry. We report for the first time that Ang II down-regulated CD62L from the surface of human neutrophils, a process which was independent of neutrophil adhesion to endothelium since neutrophils were still able to adhere to human umbilical vein endothelial cells even under doses that almost completely release CD62L from the cell surface. This process occurred through pathways involving AT1 receptors, extracellular signal-regulated kinases 1 and 2 mitogen-activated protein kinase (MAPK), phosphatidylinositol 3-kinase, and calcineurin, ruling out a role for p38 MAPK and small GTPases in the process.

      Keywords

      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribe:

      Subscribe to Atherosclerosis
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect

      References

        • Dandona P.
        • Dhindsa S.
        • Ghanim H.
        • Chaudhuri A.
        Angiotensin II and inflammation: the effect of angiotensin-converting enzyme inhibition and angiotensin II receptor blockade.
        J Hum Hypertens. 2007; 21: 20-27
        • Ross R.
        Atherosclerosis—an inflammatory disease.
        N Engl J Med. 1999; 340: 115-126
        • Ito H.
        • Takemory K.
        • Suzuki T.
        Role of angiotensin II type 1 receptor in the leukocytes and endothelial cells of brain microvessels in the pathogenesis of hypertensive cerebral injury.
        J Hypertens. 2001; 19: 591-597
        • Mazzone A.
        • De Servi S.
        • Ricevuti G.
        • et al.
        Increased expression of neutrophil and monocyte adhesion molecules in unstable coronary artery disease.
        Circulation. 1993; 88: 358-363
        • Griffin J.D.
        • Spertini O.
        • Ernst T.J.
        • et al.
        Granulocyte–macrophage colony-stimulating factor and other cytokines regulate surface expression of the leukocyte adhesion molecule-1 on human neutrophils, monocytes, and their precursors.
        J Immunol. 1990; 145: 576-584
        • Lewinsohn D.M.
        • Bargatze R.F.
        • Butcher E.C.
        Leukocyte–endothelial cell recognition: evidence of a common molecular mechanism shared by neutrophils, lymphocytes and other leukocytes.
        J Immunol. 1987; 138: 4313-4321
        • Kishimoto T.K.
        • Jutila M.A.
        • Berg E.L.
        • Butcher E.C.
        Neutrophil Mac-1 and Mel-14 adhesion proteins are inversely regulated by chemotactic factors.
        Science. 1989; 245: 1238-1241
        • Mills P.J.
        • Farag N.H.
        • Perez C.
        • Dimsdale J.E.
        Peripheral blood mononuclear cell CD62L and CD11a expression and soluble interstitial cell adhesion molecule-1 levels following infused isoproterenol in hypertension.
        J Hypertens. 2002; 20: 311-316
        • Zapolska-Downar D.
        • Siennicka A.
        • Chełstowski K.
        • et al.
        Is there an association between angiotensin-converting enzyme gene polymorphism and functional activation of monocytes and macrophages in young patients with essential hypertension?.
        J Hypertens. 2006; 24: 1565-1573
        • El Bekay R.
        • Alvarez M.
        • Monteseirín J.
        • et al.
        Oxidative stress is a critical mediator of the angiotensin II signal in human neutrophils: involvement of mitogen-activated protein kinase, calcineurin, and the transcription factor NF-κB.
        Blood. 2003; 102: 662-671
        • Spertini O.
        • Luscinskas F.W.
        • Kansas G.S.
        • et al.
        Leukocyte adhesion molecule-1 (LAM-1 L-selectin) interacts with an inducible endothelial cell ligand to support leukocyte adhesion.
        J Immunol. 1991; 147: 2565-2573
        • Monteseirín J.
        • Chacón P.
        • Vega A.
        • et al.
        L-selectin expression on neutrophils from allergic patients.
        Clin Exp Allergy. 2005; 35: 1204-1213
        • El Bekay R.
        • Alba G.
        • Reyes M.E.
        • et al.
        Rac2 GTPase activation by angiotensin II is modulated by Ca2+/calcineurin and mitogen-activated protein kinases in human neutrophils.
        J Mol Endocrinol. 2007; 39: 351-363
        • Roberts A.W.
        • Kim C.
        • Zhen L.
        • et al.
        Deficiency of the hematopoietic cell-specific Rho family GTPase Rac2 is characterized by abnormalities in neutrophil function and host defense.
        Immunity. 1999; 10: 183-196
        • Allport J.R.
        • Ding H.T.
        • Ager A.
        • Steeber D.A.
        • Tedder T.F.
        • Luscinskas F.W.
        L-selectin shedding does not regulate human neutrophil attachment, rolling, or transmigration across human vascular endothelium in vitro.
        J Immunol. 1997; 158: 4365-4372
        • Hafezi-Moghadam A.
        • Ley K.
        Relevance of L-selectin shedding for leukocyte rolling in vivo.
        J Exp Med. 1999; 189: 939-948
        • Gräfe M.
        • Auch-Schwelk W.
        • Zakrzewicz A.
        • et al.
        Angiotensin II-induced leukocyte adhesion on human coronary endothelial cells is mediated by E-selectin.
        Circ Res. 1997; 81: 804-811
        • Das U.N.
        Is angiotensin-II an endogenous pro-inflammatory molecule?.
        Med Sci Monit. 2005; 11: RA155-RA162
        • Sprinter T.A.
        Adhesion receptors of the immune system.
        Nature. 1990; 344: 425-434
        • Quinn K.
        • Henriques M.
        • Parker T.
        • Slutsky A.S.
        • Zhang H.
        Human neutrophil peptides: a novel potential mediator of inflammatory cardiovascular diseases.
        Am J Physiol Heart Circ Physiol. 2008; 295: H1817-H1824
        • Alvarez A.
        • Sanz M.J.
        Reactive oxygen species mediate angiotensin II-induced leukocyte-endothelial cell interactions in vivo.
        J Leukoc Biol. 2001; 70: 199-206
        • Prasad A.
        • Koh K.K.
        • Schenke W.H.
        • et al.
        Role of angiotensin II type 1 receptor in the regulation of cellular adhesion molecules in atherosclerosis.
        Am Heart J. 2001; 142: 248-253
        • Alvarez A.
        • Cerdá-Nicolás M.
        • Nabah Y.N.A.
        • et al.
        Direct evidence of leukocyte adhesion in arterioles by angiotensin II.
        Blood. 2004; 104: 402-408
        • Ulfman L.H.
        • Kuijper P.H.
        • van der Linden J.A.
        • Lammers J.W.
        • Zwaginga J.J.
        • Koenderman L.
        Characterization of eosinophil adhesion to TNF-alpha-activated endothelium under flow conditions: alpha 4 integrins mediate initial attachment, and E-selectin mediates rolling.
        J Immunol. 1999; 163: 343-350
        • Fraticelli A.
        • Serrano Jr., C.V.
        • Bochner B.S.
        • Capogrossi M.C.
        • Zweier J.L.
        Hydrogen peroxide and superoxide modulate leukocyte adhesion molecule expression and leukocyte–endothelial adhesion.
        Biochim Biophys Acta. 1996; 1310: 251-259
        • Jung U.
        • Ley K.
        Mice lacking two or all three selectins demonstrate overlapping and distinct functions for each selectin.
        J Immunol. 1999; 162: 6755-6762
        • Bowden R.A.
        • Ding Z.M.
        • Donnachie E.M.
        • et al.
        Role of alpha4 integrin and VCAM-1 in CD18-independent neutrophil migration across mouse cardiac endothelium.
        Circ Res. 2002; 90: 562-569
        • Stocker R.
        • Keaney Jr., J.F.
        Role of oxidative modifications in atherosclerosis.
        Physiol Rev. 2004; 84: 1381-1478
        • Jozsef L.
        • Khreiss T.
        • Fournier A.
        • Chan J.S.
        • Filep J.G.
        Extracellular signal-regulated kinase plays an essential role in endothelin-1-induced homotypic adhesion of human neutrophil granulocytes.
        Br J Pharmacol. 2002; 135: 1167-1174
        • Detmers P.A.
        • Thieblemont N.
        • Vasselon T.
        • Pironkova R.
        • Miller D.S.
        • Wright S.D.
        Potential role of membrane internalization and vesicle fusion in adhesion of neutrophils in response to lipopolysaccharide and TNF.
        J Immunol. 1996; 157: 5589-5596