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Association of monocyte subsets with vulnerability characteristics of coronary plaques as assessed by 64-slice multidetector computed tomography in patients with stable angina pectoris

      Abstract

      Objective

      The aim of the present study was to examine the relation between monocyte subsets and the presence, extent, and vulnerability characteristics of non-calcified coronary plaques (NCPs) as assessed by multidetector computed tomography (MDCT).

      Methods

      We studied 73 patients with stable angina pectoris who underwent MDCT. Two monocyte subsets (CD14+CD16 and CD14+CD16+) were measured by flow cytometry. Coronary artery plaques were assessed by 64-slice MDCT. We defined NCP vulnerability according to the presence of positive remodeling (remodeling index > 1.05) and/or low CT attenuation plaques (<35 HU).

      Results

      A total of 40 (55%) patients had identifiable vulnerable plaques. The relative proportion of CD14+CD16+ monocytes was significantly greater in patients with 1 or multiple vulnerable plaques than in patients with no vulnerable plaques or control (healthy) subjects. In addition, the relative proportion of CD14+CD16+ monocytes was positively correlated with remodeling index (r = 0.40, P < 0.01) and negatively correlated with CT attenuation value (r = −0.34, P < 0.01).

      Conclusion

      The present results suggest that an increased subset of CD14+CD16+ monocytes is related to coronary plaque vulnerability in patients with stable angina pectoris.

      Keywords

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      References

        • Geissmann F.
        • Jung S.
        • Littman D.R.
        Blood monocytes consist of two principal subsets with distinct migratory properties.
        Immunity. 2003; 19: 71-82
        • Gordon S.
        • Taylor P.R.
        Monocyte and macrophage heterogeneity.
        Nat Rev Immunol. 2005; 5: 953-964
        • Gordon S.
        Macrophage heterogeneity and tissue lipids.
        J Clin Invest. 2007; 117: 89-93
        • Shantsila E.
        • Lip G.Y.H.
        Monocytes in acute coronary syndromes.
        Arterioscler Thromb Vasc Biol. 2009; 29: 1433-1438
        • Swirski F.K.
        • Weissleder R.
        • Pittet M.J.
        Heterogeneous in vivo behavior of monocyte subsets in atherosclerosis.
        Arterioscler Thromb Vasc Biol. 2009; 29: 1424-1432
        • Mantovani A.
        • Garlanda C.
        • Locati M.
        Macrophage diversity and polarization in atherosclerosis. A question of balance.
        Arterioscler Thromb Vasc Biol. 2009; 29: 1419-1423
        • Gautier E.L.
        • Jakubzick C.
        • Randolph G.J.
        Regulation of the migration and survival of monocyte subsets by chemokine receptors and its relevance to atherosclerosis.
        Arterioscler Thromb Vasc Biol. 2009; 29: 1412-1418
        • Drevets D.A.
        • Dillon M.J.
        • Schwang J.S.
        • et al.
        The Ly-6Chigh monocyte subpopulation Listeria monocytogenes into the brain during systemic infection of mice.
        J Immunol. 2004; 172: 4418-4424
        • Sunderkotter C.
        • Nikolic T.
        • Dillon M.J.
        • et al.
        Subpopulations of mouse blood monocytes differ in maturation stage and inflammatory response.
        J Immunol. 2004; 172: 4410-4417
        • Serbina N.V.
        • Pamer E.G.
        Monocyte emigration from bone marrow during bacterial infection requires signals mediated by chemokine receptor CCR2.
        Nat Immunol. 2006; 7: 311-317
        • Tsou C.L.
        • Peters W.
        • Si Y.
        • et al.
        Critical roles for CCR2 and MCP-3 in monocyte mobilization from bone marrow and recruitment to inflammatory sites.
        J Clin Invest. 2007; 117: 902-909
        • Auffray C.
        • Fogg D.
        • Garfa M.
        • et al.
        Monitoring of blood vessels and tissues by a population of monocyte with patrolling behavior.
        Science. 2007; 317: 666-670
        • Nahrendorf M.
        • Swirski F.K.
        • Aikawa E.
        • et al.
        The healing myocardium sequentially mobilizes two monocyte subsets with divergent and complementary functions.
        J Exp Med. 2007; 204: 3037-3047
        • Leber A.W.
        • Becker A.
        • Knez A.
        • et al.
        Accuracy of 64-slice computed tomography to classify and quantify plaque volumes in the proximal coronary system: a comparative study using intravascular ultrasound.
        J Am Coll Cardiol. 2006; 47: 672-677
        • Kitagwa T.
        • Yamamoto H.
        • Ohhashi N.
        • et al.
        Comprehensive evaluation of non-calcified coronary plaque characteristics detected using 64-slice computed tomography in patients with proven or suspected coronary artery disease.
        Am Heart J. 2007; 154: 1191-1198
        • Kitagawa T.
        • Yamamoto H.
        • Horiguchi J.
        • et al.
        Characterization of noncalcified coronary plaques and identification of culprit lesions in patients with acute coronary syndrome by 64-slice computed tomography.
        JACC Cardiovasc Imaging. 2009; 2: 153-160
        • Pundziute G.
        • Schuijf J.D.
        • Jukema J.W.
        • et al.
        Prognostic value of multislice computed tomography coronary angiography in patients with known or suspected coronary artery disease.
        J Am Coll Cardiol. 2007; 49: 62-70
        • Tsujioka H.
        • Imanishi T.
        • Ikejima H.
        • et al.
        Impact of heterogeneity of human peripheral blood monocyte subsets on myocardial salvage in patients with primary acute myocardial infarction.
        J Am Coll Cardiol. 2009; 54: 130-138
        • Steppich B.
        • Dayyani F.
        • Gruber R.
        • Lorenz R.
        • Mack M.
        • Ziegler-Heitbrock H.W.
        Selective mobilization of CD14+CD16+ monocytes by exercise.
        Am J Physiol. 2000; 279: C578-C586
        • Kashiwagi M.
        • Tanaka A.
        • Kitabata H.
        • et al.
        Non-invasive assessment of thin-cap fibroatheroma by multidetector computed tomography.
        JACC Cardiovasc Imaging. 2009; 2: 1412-1419

      .

        • Huang Z.S.
        • Wang C.H.
        • Yip P.K.
        • yang C.Y.
        • Lee T.K.
        In hypercholesterolemia, lower peripheral monocyte count is unique among the major predictors of atherosclerosis.
        Arterioscler Thromb Vasc Biol. 1996; 16: 256-261
        • Nasir K.
        • Guallar E.
        • Navas-Acien A.
        • Criqui M.H.
        • Lima J.A.
        Relationship of monocyte count and peripheral disease: results from the National Health and Nutrition Examination Survey 1999–2002.
        Arterioscler Thromb Vasc Biol. 2005; 25: 1966-1971
        • Horne B.D.
        • Anderson J.L.
        • John J.M.
        • et al.
        Which white blood cell subtypes predict increased cardiovascular risk?.
        J Am Coll Cardiol. 2005; 45: 1638-1643
        • Olivares R.
        • Ducimetiere P.
        • Claude J.R.
        Monocyte count: a risk factor for coronary heart disease?.
        Am J Epidemiol. 1993; 137: 49-53
        • Swirski F.K.
        • Libby P.
        • Aikawa E.
        • et al.
        Ly-6Chi monocytes dominate hypercholesterolemia-associated monocytosis and give rise to macrophages in atheroma.
        J Clin Invest. 2007; 117: 195-205
        • Schlitt A.
        • Heine G.H.
        • Blankenberg S.
        • et al.
        CD14+CD16+ monocytes in coronary artery disease and their relationship to serum TNF-alpha levels.
        Thromb Haemost. 2004; 92: 419-424
        • Roethe G.
        • Garbiel H.
        • Kovacs E.
        • et al.
        Peripheral blood mononuclear phagocyte subpopulations as cellular markers in hypercholesterolemia.
        Arterioscler Thromb Vasc Biol. 1996; 16: 1437-1447
        • Moatti D.
        • Faure S.
        • Fumeron F.
        • et al.
        Polymorphism in the fractalkine receptor CX3CR1 as a genetic risk factor in the coronary artery disease.
        Blood. 2001; 97: 1925-1928
        • MacDermott D.H.
        • Halcox J.P.J.
        • Schenke W.H.
        • et al.
        Association between polymorphism in the chemokine receptor CX3CR1 and coronary vascular endothelial dysfunction and atherosclerosis.
        Circ Res. 2001; 89: 401-407
        • Motoyama S.
        • Kondo T.
        • Sarai M.
        • et al.
        Multislice computed tomographic characteristics of coronary lesions in acute coronary syndromes.
        J Am Coll Cardiol. 2007; 50: 319-326
        • Hoffmann U.
        • Moselewski F.
        • Nieman K.
        • et al.
        Noninvasive assessment of plaque morphology and composition in culprit and stable lesions in acute coronary syndrome and stable lesions in stable angina by multidetector computed tomography.
        J Am Coll Cardiol. 2006; 47: 1655-1662
        • Motoyama S.
        • Sarai M.
        • Harigaya H.
        • et al.
        Computed tomographic angiography characteristics of atherosclerotic plaques subsequently resulting in acute coronary syndrome.
        J Am Coll Cardiol. 2009; 54: 49-57
        • Liuzzo G.
        • Biasucci L.M.
        • Gallimore J.R.
        • et al.
        The prognostic value of C-reactive protein and serum amyloid A protein in severe unstable angina.
        N Engl J Med. 1994; 331: 417-424
        • Biasucci L.M.
        • Vitelli A.
        • Liuzzo G.
        • et al.
        Elevated levels of interleukin-6 in unstable angina.
        Circulation. 1996; 94: 874-877
        • Liuzzo G.
        • Biasucci L.M.
        • Rebuzzi A.G.
        • et al.
        Plasma protein acute-phase response in unstable angina is not induced by ischemic injury.
        Circulation. 1996; 94: 2373-2380
        • Heslop C.L.
        • Frohlich J.L.
        • Hill J.S.
        • Myeloperoxidase
        C-reactive protein have combined utility for long-term prediction of cardiovascular mortality after coronary angiography.
        J Am Coll Cardiol. 2010; 55: 1102-1109
        • Meuwese M.C.
        • Stroes E.S.G.
        • Hazen S.L.
        • et al.
        Serum myeloperoxidase levels are associated with the future risk of coronary artery disease in apparently healthy individuals.
        J Am Coll Cardiol. 2007; 50: 159-165
        • Wong N.D.
        • Gransar H.
        • Narula J.
        • et al.
        Myeloperoxidase, subclinical atherosclerosis, and cardiovascular disease events.
        J Am Coll Cardiol Img. 2009; 2: 1093-1099