Abstract
Objective
Oxidative stress is a main mechanism involved in vascular pathologies. Increased thioredoxin
(TRX) levels have been observed in several oxidative stress-associated cardiovascular
diseases. We aim to test the potential role of TRX as a biomarker of oxidative stress
in abdominal aortic aneurysm (AAA).
Methods
TRX levels were analysed in both AAA intraluminal thrombus (ILT) tissue and in tissue-conditioned
media by immunohistochemistry, Western blot and ELISA. Moreover, serum TRX levels
were assessed in AAA Caucasian patients by ELISA.
Results
TRX was mainly localized in the luminal part of ILT in AAA. Compared with the abluminal
layer, TRX release was increased in the luminal layer of the ILT of AAA (31 ± 9 ng/ml vs. 9 ± 3 ng/ml, p < 0.05). The interest of this approach is that we can identify proteins potentially
released into the blood compartment, which could serve as biomarkers of the pathology.
In a training population, serum TRX levels were significantly increased in patients
with AAA relative to healthy subjects (50 ± 6 ng/ml vs. 26 ± 3 ng/ml, p < 0.05). These results were validated in a second independent group of patients. Moreover,
a positive correlation between TRX and AAA size (rho = 0.5, p < 0.001) was observed. Finally, in AAA samples with follow-up, TRX was positively associated
to aneurismal growth rate (rho = 0.25, p = 0.027).
Conclusions
TRX release is increased in the luminal part of AAA and TRX serum levels are increased
in AAA patients compared with healthy subjects. TRX levels correlates with AAA size
and expansion, suggesting its potential role as a biomarker of AAA evolution.
Abbreviations:
TRX (thioredoxin), AAA (abdominal aortic aneurysm), RBCs (red blood cells), PMNs (polymorphonuclear neutrophils), MPO (myeloperoxidase), MIF (macrophage inhibitory factor)Keywords
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Article info
Publication history
Published online: July 08, 2010
Accepted:
May 19,
2010
Received in revised form:
May 11,
2010
Received:
January 9,
2010
Identification
Copyright
© 2010 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.
