Highlights
- •The proatherogenic apoB-containing lipoprotein-bound Lp-PLA2 activity was significantly reduced after bariatric surgery.
- •Bariatric surgery reduced the concentration of circulating oxidised LDL in plasma.
- •A switch towards predominantly larger HDL particles was observed in all patients after surgery.
Abstract
Objective
The purpose of this study was to evaluate the effect of weight loss induced in morbidly
obese subjects by Roux-en-Y gastric bypass bariatric surgery on the atherogenic features
of their plasma lipoproteins.
Methods
Twenty-one morbidly obese subjects undergoing bariatric surgery were followed up for
up to 1 year after surgery. Plasma and lipoproteins were assayed for chemical composition
and lipoprotein-associated phospholipase A2 (Lp-PLA2) activity. Lipoprotein size was
assessed by non-denaturing polyacrylamide gradient gel electrophoresis, and oxidised
LDL by ELISA. Liver samples were assayed for mRNA abundance of oxidative markers.
Results
Lipid profile analysis revealed a reduction in the plasma concentrations of cholesterol
and triglycerides, which were mainly associated with a significant reduction in the
plasma concentration of circulating apoB-containing lipoproteins rather than with
changes in their relative chemical composition. All patients displayed a pattern A
phenotype of LDL subfractions and a relative increase in the antiatherogenic plasma
HDL-2 subfraction (>2-fold; P < 0.001). The switch towards predominantly larger HDL particles was due to an increase
in their relative cholesteryl ester content. Excess weight loss also led to a significant
decrease in the plasma concentration of oxidised LDL (∼−25%; P < 0.01) and in the total Lp-PLA2 activity. Interestingly, the decrease in plasma
Lp-PLA2 was mainly attributed to a decrease in the apoB-containing lipoprotein-bound
Lp-PLA2.
Conclusion
Our data indicate that the weight loss induced by bariatric surgery ameliorates the
atherogenicity of plasma lipoproteins by reducing the apoB-containing Lp-PLA2 activity
and oxidised LDL, as well as increasing the HDL-2 subfraction.
Keywords
Abbreviations:
apo (apolipoprotein), HDL (high-density lipoprotein), HDL-C (HDL cholesterol), LDL (low-density lipoprotein), LDL-C (LDL cholesterol), Lp-PLA2 (lipoprotein-associated phospholipase A2), MSR1 (scavenger receptor A (SRA) gene), non-HDL-C (non-HDL cholesterol), OLR1 (oxidised (lectin-like) LDL receptor-1 gene), oxLDL (oxidised LDL), PLA2G7 (group VII phospholipase A2 gene), PON1 (plasma paraoxonase-(PON)1 gene), RYGBP (Roux-en-Y gastric bypass), SCARB1 (scavenger receptor B type I (SR-BI) gene), VLDL (very-low-density lipoprotein)To read this article in full you will need to make a payment
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Article info
Publication history
Published online: March 13, 2014
Accepted:
February 17,
2014
Received in revised form:
February 14,
2014
Received:
November 26,
2013
Identification
Copyright
© 2014 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.
