33 - Cholesterol efflux and reverse cholesterol transport| Volume 235, ISSUE 2, e182, August 2014

Autophagy is not required for abca1-mediated cholesterol efflux in cholesterol loaded human monocyte-derived macrophages

      Objectives: The accumulation of cholesteryl ester (CE)-rich lipid droplets (LD) is a hallmark feature of foam cells formation in atherosclerosis. The ABCA1-mediated efflux of free cholesterol (FC) is crucial for removal of excess cholesterol. Using cholesterol-loaded primary bone marrow- derived and peritoneal mouse macrophages, Yves Marcel's team reported that autophagy mediated the delivery of cytosolic LD to lysosomes and that Acid CE Hydrolases (aCEH) degrades LD-CE to generate FC for efflux. Our goal was to compare the involvement of autophagy in cholesterol efflux between lipid-loaded J774 mouse macrophage cell line and primary cultures of human monocyte-derived macrophages (HMDM).
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