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Nebivolol to attenuate the effects of hyper-homocysteinaemia in rats

      Highlights

      • Four week duration of hyperhomocysteinemia (hHcy) caused significant oxidative stress in brain, kidney, liver, heart tissues.
      • Nebivolol attenuated the oxidative stress in brain, kidney, liver and heart tissues that is caused by hHcy.
      • Four week duration of intermediate hHcy caused vascular structural changes (outward vascular remodelling) in the aorta.
      • Nebivolol prevented aorta against hHcy induced structural changes (especially vascular outward remodelling).
      • Nebivolol slightly decreased the serum levels of homocysteine, and may also be useful to prevent hyperhomocysteinemia.

      Abstract

      Objective

      This study investigated the prophylactic effect of nebivolol against hyper-homocysteinaemia (hHcy) induced oxidative stress in brain, heart, liver and kidney tissues and histomorphometric changes in the thoracic aorta.

      Methods

      Twenty-four adult male Wistar rats were divided into a control, nebivolol, hHcy and nebivolol + hHcy group. hHcy was induced by oral administration of l-methionine (1 g/kg/day) for 28 days. 10 mg/kg/day nebivolol was administered orally for 28 days. Malondialdehyde (MDA) and glutathione (GSH) levels and catalase (CAT) and superoxide dismutase (SOD) activities in the tissues were determined. The total cross-sectional area (TCSA), luminal cross-sectional area (LCSA) and intima-media thickness (IMT) were measured in the thoracic aorta.

      Results

      Homocysteine (Hcy) levels were lower in the nebivolol + hHcy group than in the hHcy group. Nebivolol treatment significantly decreased high MDA levels in the brain, heart and liver tissues. The level of GSH was higher in the brain, heart and kidney tissues of the nebivolol + hHcy group (P < 0.001). The activity of CAT increased only in the kidney tissue of the nebivolol + hHcy group (P < 0.01), and the activity of SOD was significantly increased in all the tissues in this group. Increased TCSA and IMT in the nebivolol + hHcy group were significantly decreased after nebivolol administration. The LCSA was significantly higher in the hHcy group than the control group, probably due to outward vascular remodelling.

      Conclusion

      Nebivolol treatment may be useful in different clinical scenarios where hHcy affects physiopathological pathways.

      Keywords

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