Advertisement
Research Article| Volume 241, ISSUE 1, P255-258, July 2015

Macrophage subsets in the adipose tissue could be modified by sex and the reproductive age of women

      Highlights

      • We analysed proinflammatory monocytes/macrophages in perirenal and perivascular fat.
      • We found no differences in the monocyte/macrophage content between men and women.
      • The highest proportion of double positive CD14+CD16+ cells was found in older women.
      • Correlation of monocyte/macrophage content with age was found only in older women.
      • CD14+CD16+ monocytes/macrophages should be evaluated according to the sex and age.

      Abstract

      The presence of proinflammatory monocytes/macrophages (CD14+CD16+) has been documented in conditions of inflammation, such as atherosclerosis. We analysed the proportion of proinflammatory monocytes/macrophages in perirenal and perivascular fat in healthy living kidney donors with regard to sex and age reflecting reproductive status in women; therefore, women were further divided to younger and older group (younger and older than 51 years) reflecting potential age of menopause. Monocyte/macrophages were identified as CD14+ mononuclear cells and divided into subpopulations based on the co-expression of CD16. We found no differences in the monocyte/macrophage content between men (n = 15) and women (n = 28). Conversely, we observed a higher proportion of double positive CD14+CD16+ monocytes/macrophages in older women (n = 14) compared to younger women (n = 14). In addition, a strong correlation was found between the monocyte/macrophage content in fat and age only in older women. Therefore, proinflammatory monocytes/macrophages (CD14+CD16+) should be evaluated according to the sex and age.

      Keywords

      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribe:

      Subscribe to Atherosclerosis
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect

      References

        • Lejsková M.
        • Alušík S.
        • Valenta Z.
        • Adámková S.
        • Piťha J.
        Natural postmenopause is associated with an increase in combined cardiovascular risk factors.
        Physiol. Res. 2012; 61: 587-596
        • Lejsková M.
        • Alušík S.
        • Suchánek M.
        • Zecová S.
        • Pitha J.
        Menopause: clustering of metabolic syndrome components and population changes in insulin resistance.
        Climacteric. 2011; 14: 83-91
        • Cifkova R.
        • Pitha J.
        • Lejskova M.
        • Lanska V.
        • Zecova S.
        Blood pressure around the menopause: a population study.
        J. Hypertens. 2008; 26: 1976-1982
        • Curat C.A.
        • Miranville A.
        • Sengene's C.
        • Diehl M.
        • Tonus C.
        • Busse R.
        • Bouloumie A.
        From blood monocytes to adipose tissue–resident macrophages induction of diapedesis by human mature adipocytes.
        Diabetes. 2004; 53: 1285-1292
        • Surmi B.K.
        • Hasty A.H.
        The role of chemokines in recruitment of immune cells to the artery wall and adipose tissue.
        Vasc. Pharmacol. 2010; 52: 27-36
        • Suganami T.
        • Ogawa Y.
        Adipose tissue macrophages: their role in adipose tissue remodeling.
        J. Leukoc. Biol. 2010; 88: 1-7
        • Fingerle G.
        • Pforte A.
        • Passlick B.
        • Blumenstein M.
        • Strobel M.
        • Ziegler-Heitbrock H.W.
        The novel subset of CD14+/CD16+ blood monocytes is expanded in sepsis patients.
        Blood. 1993; 82: 3170-3176
        • Koch S.
        • Kucharzik T.
        • Heidemann J.
        • Nusrat A.
        • Luegering A.
        Investigating the role of proinflammatory CD16+ monocytes in the pathogenesis of inflammatory bowel disease.
        Clin. Exp. Immunol. 2010; 161: 332-341
        • Pamukcu B.
        • Lip G.Y.
        • Devitt A.
        • Griffiths H.
        • Shantsila E.
        The role of monocytes in atherosclerotic coronary artery disease.
        Ann. Med. 2010; 42: 394-403
        • Belge K.U.
        • Dayyani F.
        • Horelt A.
        • Siedlar M.
        • Frankenberger M.
        • Frankenberger B.
        • Espevik T.
        • Ziegler-Heitbrock L.
        The proinflammatory CD14+CD16+DR++ monocytes are a major source of TNF.
        J. Immunol. 2002; 168: 3536-3542
        • Zawada A.M.
        • Rogacev K.S.
        • Rotter B.
        • Winter P.
        • Marell R.R.
        • Fliser D.
        • Heine G.H.
        SuperSAGE evidence for CD14++CD16+ monocytes as a third monocyte subset.
        Blood. 2011; 118: e50-e61
        • Cífková R.
        • Skodová Z.
        • Bruthans J.
        • Adámková V.
        • Jozífová M.
        • Galovcová M.
        • Wohlfahrt P.
        • Krajcoviechová A.
        • Poledne R.
        • Stávek P.
        • Lánská V.
        Longitudinal trends in major cardiovascular risk factors in the Czech population between 1985 and 2007/8. Czech MONICA and Czech post-MONICA.
        Atherosclerosis. 2010; 211: 676-681
        • Kralova Lesna I.
        • Suchanek P.
        • Brabcova E.
        • Kovar J.
        • Malinska H.
        • Poledne R.
        Effect of different types of dietary fatty acids on subclinical inflammation in humans.
        Physiol. Res. 2013; 62: 145-152
        • Poledne R.
        • Lorenzová A.
        • Stávek P.
        • Valenta Z.
        • Hubácek J.
        • Suchánek P.
        • Pitha J.
        Proinflammatory status, genetics and atherosclerosis.
        Physiol Res. 2009; 58: S111-s118
        • Tintera J.
        • Harantová P.
        • Suchánek P.
        • Dvoráková A.
        • Adamová M.
        • Hájek M.
        • Poledne R.
        Quantification of intra-abdominal fat during controlled weight reduction: assessment using the water-suppressed breath-hold MRI technique.
        Physiol. Res. 2004; 53: 229-234
        • Soltis E.E.
        • Cassis L.A.
        Influence of perivascular adipose tissue on rat aortic smooth muscle responsiveness.
        Clin. Exp. Hypertens. A. 1991; 13: 277-296
        • Rajsheker S.
        • Manka D.
        • Blomkalns A.L.
        • Chatterjee T.K.
        • Stoll L.L.
        • Weintraub N.L.
        Crosstalk between perivascular adipose tissue and blood vessels.
        Curr. Opin. Pharmacol. 2010; 10: 191-196
        • Szasz T.
        • Webb R.C.
        Perivascular adipose tissue: more than just structural support.
        Clin. Sci. (Lond). 2012; 122: 1-12
        • Bourlier V.
        • Zakaroff-Girard A.
        • Miranville A.
        • De Barros S.
        • Maumus M.
        • Sengenes C.
        • Galitzky J.
        • Lafontan M.
        • Karpe F.
        • Frayn K.N.
        • Bouloumié A.
        Remodeling phenotype of human subcutaneous adipose tissue macrophages.
        Circulation. 2008; 117: 806-815
        • Chawla A.
        • Nguyen K.D.
        • Goh Y.P.
        Macrophage-mediated inflammation in metabolic disease.
        Nat. Rev. Immunol. 2011; 11: 738-749
        • Lehman S.J.
        • Massaro J.M.
        • Schlett C.L.
        • O'Donnel C.J.
        • Hoffmann U.
        • Fox C.S.
        Peri-aortic fat, cardiovascular disease risk factors, and aortic calcification: the Framingham heart study.
        Atherosclerosis. 2010; 210: 656-661
        • Greif M.
        • Becker A.
        • von Ziegler F.
        • Lebherz C.
        • Lehrke M.
        • Broedl U.C.
        • Tittus J.
        • Parhofer K.
        • Becker C.
        • Reiser M.
        • Knez A.
        • Leber A.W.
        Pericardial adipose tissue determined by dual source CT is a risk factor for coronary atherosclerosis.
        Arterioscler. Thromb. Vasc. Biol. 2009; 29: 781-786
        • Buechler C.
        • Eisinger K.
        • Krautbauer S.
        Diagnostic and prognostic potential of the macrophage specific receptor CD163 in inflammatory diseases.
        Inflamm. Allergy Drug Targets. 2013; 12: 391-402
        • Murray P.J.
        • Allen J.E.
        • Biswas S.K.
        • Fisher E.A.
        • Gilroy D.W.
        • Goerdt S.
        • Gordon S.
        • Hamilton J.A.
        • Ivashkiv L.B.
        • Lawrence T.
        • Locati M.
        • Mantovani A.
        • Martinez F.O.
        • Mege J.L.
        • Mosser D.M.
        • Natoli G.
        • Saeij J.P.
        • Schultze J.L.
        • Shirey K.A.
        • Sica A.
        • Suttles J.
        • Udalova I.
        • van Ginderachter J.A.
        • Vogel S.N.
        • Wynn T.A.
        Macrophage activation and polarization: nomenclature and experimental guidelines.
        Immunity. 2014; 41: 14-20

      Linked Article

      • Importance of sex and gender in atherosclerosis and cardiovascular disease
        AtherosclerosisVol. 241Issue 1
        • Preview
          In this special issue of the journal, there are papers on bone health and coronary artery calcification, age and sex differences in the effect of parental stroke on the progression of carotid intima-media thickness, macrophage subsets in the adipose tissue by sex and by reproductive age of women, uric acid levels and metabolic syndrome, sex differences in cardiovascular risk factors and disease prevention, severity of stable coronary artery disease and its biomarkers, cardiovascular disease and autoimmune diseases genetics of cardiovascular disease, outcome after CABG; association of serum phosphorus with subclinical atherosclerosis in chronic kidney disease and relationship of uric acid levels to coronary disease.
        • Full-Text
        • PDF
      • Sex matters to the heart: A special issue dedicated to the impact of sex related differences of cardiovascular diseases
        AtherosclerosisVol. 241Issue 1
        • Preview
          Ever since the early 1980s most cardiovascular research has focused on men [1]. This phenomenon has led to the under appreciation of sex-differences in cardiovascular disease (CVD) from an etiological, prognostic, diagnostic and therapeutic perspective. Several initiatives to promote women's health, such as the Women's Health Initiative [2] have been initiated and have changed the practice of cardiovascular disease prevention in women over the past decade. This ultimately led to the first guidelines for cardiovascular disease prevention in women by the American Heart Association in 1999 [3].
        • Full-Text
        • PDF