MCSF-1 induces differentiation of monocytes into “Mφ-2-like” macrophages and plays
a role in the monocyte-to-macrophage transition within plaques. Here we have analysed
changes in gene expression between monocytes from 458 healthy subjects and paired,
monocyte-derived macrophages cultured for 7 days with M-CSF1 (50μg.L-1) in macrophage serum-free medium (Invitrogen). Gene expression was analysed on Illumina’s
Human Ref-8 arrays, which contained 24,526 probes corresponding to 18,311 distinct
genes. Patterns of gene expression were analyzed using QUSAGE software linked to the
Reactome database to identify key pathways. Pathways were excluded if <3 or <40% of
genes were detected, or if the mean log-fold change was >0.3 or <-0.3.
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Publication history
EAS-0365
Identification
Copyright
© 2015 Published by Elsevier Inc.
