Abstract| Volume 241, ISSUE 1, e81, July 2015

Transcriptional profiling of the human M-CSF-1-driven monocyte-macrophage transition identifies genes regulating cholesterol efflux and synthesis

      MCSF-1 induces differentiation of monocytes into “Mφ-2-like” macrophages and plays a role in the monocyte-to-macrophage transition within plaques. Here we have analysed changes in gene expression between monocytes from 458 healthy subjects and paired, monocyte-derived macrophages cultured for 7 days with M-CSF1 (50μg.L-1) in macrophage serum-free medium (Invitrogen). Gene expression was analysed on Illumina’s Human Ref-8 arrays, which contained 24,526 probes corresponding to 18,311 distinct genes. Patterns of gene expression were analyzed using QUSAGE software linked to the Reactome database to identify key pathways. Pathways were excluded if <3 or <40% of genes were detected, or if the mean log-fold change was >0.3 or <-0.3.
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