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Genomic variant in CAV1 increases susceptibility to coronary artery disease and myocardial infarction

  • Author Footnotes
    1 These authors contributed equally to this work.
    Shanshan Chen
    Footnotes
    1 These authors contributed equally to this work.
    Affiliations
    Key Laboratory of Molecular Biophysics of the Ministry of Education, Cardio-X Institute, College of Life Science and Technology and Center for Human Genome Research, Huazhong University of Science and Technology, Wuhan, China
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  • Author Footnotes
    1 These authors contributed equally to this work.
    Xiaojing Wang
    Footnotes
    1 These authors contributed equally to this work.
    Affiliations
    Key Laboratory of Molecular Biophysics of the Ministry of Education, Cardio-X Institute, College of Life Science and Technology and Center for Human Genome Research, Huazhong University of Science and Technology, Wuhan, China
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  • Author Footnotes
    1 These authors contributed equally to this work.
    Junhan Wang
    Footnotes
    1 These authors contributed equally to this work.
    Affiliations
    Department of Clinical Laboratory, University Hospital, Huazhong University of Science and Technology, Wuhan, China
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  • Author Footnotes
    1 These authors contributed equally to this work.
    Yuanyuan Zhao
    Footnotes
    1 These authors contributed equally to this work.
    Affiliations
    Key Laboratory of Molecular Biophysics of the Ministry of Education, Cardio-X Institute, College of Life Science and Technology and Center for Human Genome Research, Huazhong University of Science and Technology, Wuhan, China
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  • Author Footnotes
    1 These authors contributed equally to this work.
    Dan Wang
    Footnotes
    1 These authors contributed equally to this work.
    Affiliations
    Key Laboratory of Molecular Biophysics of the Ministry of Education, Cardio-X Institute, College of Life Science and Technology and Center for Human Genome Research, Huazhong University of Science and Technology, Wuhan, China
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  • Author Footnotes
    1 These authors contributed equally to this work.
    Chengcheng Tan
    Footnotes
    1 These authors contributed equally to this work.
    Affiliations
    Key Laboratory of Molecular Biophysics of the Ministry of Education, Cardio-X Institute, College of Life Science and Technology and Center for Human Genome Research, Huazhong University of Science and Technology, Wuhan, China
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  • Author Footnotes
    1 These authors contributed equally to this work.
    Jingjing Fa
    Footnotes
    1 These authors contributed equally to this work.
    Affiliations
    Key Laboratory of Molecular Biophysics of the Ministry of Education, Cardio-X Institute, College of Life Science and Technology and Center for Human Genome Research, Huazhong University of Science and Technology, Wuhan, China
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  • Rongfeng Zhang
    Affiliations
    Department of Cardiology, First Affiliated Hospital of Dalian Medical University, Dalian, China
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  • Fan Wang
    Affiliations
    Key Laboratory of Molecular Biophysics of the Ministry of Education, Cardio-X Institute, College of Life Science and Technology and Center for Human Genome Research, Huazhong University of Science and Technology, Wuhan, China
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  • Chaoping Xu
    Affiliations
    Key Laboratory of Molecular Biophysics of the Ministry of Education, Cardio-X Institute, College of Life Science and Technology and Center for Human Genome Research, Huazhong University of Science and Technology, Wuhan, China
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  • Yufeng Huang
    Affiliations
    Key Laboratory of Molecular Biophysics of the Ministry of Education, Cardio-X Institute, College of Life Science and Technology and Center for Human Genome Research, Huazhong University of Science and Technology, Wuhan, China
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  • Sisi Li
    Affiliations
    Key Laboratory of Molecular Biophysics of the Ministry of Education, Cardio-X Institute, College of Life Science and Technology and Center for Human Genome Research, Huazhong University of Science and Technology, Wuhan, China
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  • Dan Yin
    Affiliations
    Key Laboratory of Molecular Biophysics of the Ministry of Education, Cardio-X Institute, College of Life Science and Technology and Center for Human Genome Research, Huazhong University of Science and Technology, Wuhan, China
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  • Xin Xiong
    Affiliations
    Key Laboratory of Molecular Biophysics of the Ministry of Education, Cardio-X Institute, College of Life Science and Technology and Center for Human Genome Research, Huazhong University of Science and Technology, Wuhan, China
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  • Xiuchun Li
    Affiliations
    Key Laboratory of Molecular Biophysics of the Ministry of Education, Cardio-X Institute, College of Life Science and Technology and Center for Human Genome Research, Huazhong University of Science and Technology, Wuhan, China
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  • Qiuyun Chen
    Affiliations
    Center for Cardiovascular Genetics, Department of Molecular Cardiology, Cleveland Clinic, Cleveland, OH 44195, USA

    Department of Molecular Medicine, CCLCM, Department of Genetics and Genome Sciences, Case Western Reserve University, Cleveland, OH 44195, USA
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  • Xin Tu
    Affiliations
    Key Laboratory of Molecular Biophysics of the Ministry of Education, Cardio-X Institute, College of Life Science and Technology and Center for Human Genome Research, Huazhong University of Science and Technology, Wuhan, China
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  • Yanzong Yang
    Affiliations
    Department of Cardiology, First Affiliated Hospital of Dalian Medical University, Dalian, China
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  • Yunlong Xia
    Affiliations
    Department of Cardiology, First Affiliated Hospital of Dalian Medical University, Dalian, China
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  • Chengqi Xu
    Affiliations
    Key Laboratory of Molecular Biophysics of the Ministry of Education, Cardio-X Institute, College of Life Science and Technology and Center for Human Genome Research, Huazhong University of Science and Technology, Wuhan, China
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  • Qing K. Wang
    Correspondence
    Corresponding author. College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
    Affiliations
    Key Laboratory of Molecular Biophysics of the Ministry of Education, Cardio-X Institute, College of Life Science and Technology and Center for Human Genome Research, Huazhong University of Science and Technology, Wuhan, China

    Center for Cardiovascular Genetics, Department of Molecular Cardiology, Cleveland Clinic, Cleveland, OH 44195, USA

    Department of Molecular Medicine, CCLCM, Department of Genetics and Genome Sciences, Case Western Reserve University, Cleveland, OH 44195, USA
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  • Author Footnotes
    1 These authors contributed equally to this work.

      Highlights

      • Identification of a new susceptibility gene (CAV1) for CAD and MI.
      • First demonstration of association between a genomic variant in CAV1 and CAD/MI.
      • The minor allele A of CAV1 tagSNP rs3807989 increases risk of CAD and MI.
      • Allele A of rs3807989 is associated with increased CAV1 expression, but not CAV2 expression.

      Abstract

      Background

      The CAV1 gene encodes caveolin-1 expressed in cell types relevant to atherosclerosis. Cav-1-null mice showed a protective effect on atherosclerosis under the ApoE−/− background. However, it is unknown whether CAV1 is linked to CAD and MI in humans. In this study we analyzed a tagSNP for CAV1 in intron 2, rs3807989, for potential association with CAD.

      Methods and Results

      We performed case–control association studies in three independent Chinese Han populations from GeneID, including 1249 CAD cases and 841 controls in Population I, 1260 cases and 833 controls in Population II and 790 cases and 1212 controls in Population III (a total of 3299 cases and 2886 controls). We identified significant association between rs3807989 and CAD in three independent populations and in the combined population (Padj = 2.18 × 10−5, OR = 1.19 for minor allele A). We also detected significant association between rs3807989 and MI (Padj = 5.43 × 10−5, OR = 1.23 for allele A). Allele A of SNP rs3807989 was also associated with a decreased level of LDL cholesterol. Although rs3807989 is a tagSNP for both CAV1 and nearby CAV2, allele A of SNP rs3807989 was associated with an increased expression level of CAV1 (both mRNA and protein), but not CAV2.

      Conclusions

      The data in this study demonstrated that rs3807989 at the CAV1/CAV2 locus was associated with significant risk of CAD and MI by increasing expression of CAV1 (but not CAV2). Thus, CAV1 becomes a strong candidate susceptibility gene for CAD/MI in humans.

      Keywords

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