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Atherosclerotic dyslipidemia revealed by plasma lipidomics on ApoE−/− mice fed a high-fat diet

  • Author Footnotes
    1 These authors contributed equally to this work.
    Yanyan Chen
    Footnotes
    1 These authors contributed equally to this work.
    Affiliations
    Institute of Traditional Chinese Medicine Research, Tianjin University of Traditional Chinese Medicine, Tianjin, PR China

    Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, Jinan University, Guangzhou, PR China
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  • Author Footnotes
    1 These authors contributed equally to this work.
    Shiyuan Wen
    Footnotes
    1 These authors contributed equally to this work.
    Affiliations
    Institute of Traditional Chinese Medicine Research, Tianjin University of Traditional Chinese Medicine, Tianjin, PR China

    Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, Jinan University, Guangzhou, PR China
    Search for articles by this author
  • Miaomiao Jiang
    Correspondence
    Corresponding author. 88 Yuquan Road, Nankai District, Tianjin, PR China.
    Affiliations
    Institute of Traditional Chinese Medicine Research, Tianjin University of Traditional Chinese Medicine, Tianjin, PR China
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  • Yan Zhu
    Affiliations
    Institute of Traditional Chinese Medicine Research, Tianjin University of Traditional Chinese Medicine, Tianjin, PR China
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  • Liqin Ding
    Affiliations
    Institute of Traditional Chinese Medicine Research, Tianjin University of Traditional Chinese Medicine, Tianjin, PR China
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  • Hong Shi
    Affiliations
    Institute of Traditional Chinese Medicine Research, Tianjin University of Traditional Chinese Medicine, Tianjin, PR China
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  • Pengzhi Dong
    Affiliations
    Institute of Traditional Chinese Medicine Research, Tianjin University of Traditional Chinese Medicine, Tianjin, PR China
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  • Jing Yang
    Affiliations
    Institute of Traditional Chinese Medicine Research, Tianjin University of Traditional Chinese Medicine, Tianjin, PR China
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  • Yue Yang
    Affiliations
    Institute of Traditional Chinese Medicine Research, Tianjin University of Traditional Chinese Medicine, Tianjin, PR China
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  • Author Footnotes
    1 These authors contributed equally to this work.

      Highlights

      • PCs, SP and SMs are the most altered lipid species in the ApoE−/− model.
      • PCs with high saturation and SMs tend to correlate with TC and LDL-C levels.
      • Dyslipidemia is possibly associated with sphingolipid and glycerophospholipid metabolism.

      Abstract

      Background and aims

      The pathological process of atherosclerosis is closely related to lipid accumulation at arterial wall caused by lipoproteins transporting lipids through blood. In this study, we investigate the lipid composition in the plasma of apolipoprotein E deficient (ApoE−/−) mice fed a high-fat diet to reveal atherosclerosis-induced dyslipidemia.

      Methods

      ApoE−/− and corresponding wild-type C57BL/6J mice were used as the pathology model and control, respectively, and were fed a high-fat or normal diet. Lipidomics approach based on chromatography coupled with time of fight mass spectrometry (UPLC-Q/TOF-MS) was applied to profile lipid species.

      Results

      Discrimination analysis revealed that 1 lysophosphatidylcholine (LPC) and 6 phosphatidylcholines (PCs) were identified to distinguish C57BL/6J mice fed a normal and high-fat diet. PC (16:0/18:1) and PC (18:0/18:1) were also extracted when the comparison was done between ApoE−/− and C57BL/6J mice, both fed a high-fat diet. Besides the 2 PCs, the other 4 PCs, 1 sphinganine (SP) and 3 sphingomyelins (SMs) were identified in the second comparing case, among which PC (16:0/16:0), PC (18:0/16:1), SM (d16:0/28:5), SM (d18:1/24:1) and SM (d18:1/16:0) showed obviously positive correlations with the plasma levels of TC and LDL-C. However, no significant relationship was observed between the differential lipids and TG or HDL-C.

      Conclusions

      This study reveals that SP, SMs and PCs are the particularly changed lipid species induced by atherosclerotic lesions in the ApoE-/- model, indicating a disturbance on sphingolipid and glycerophospholipid metabolism during the progression of atherosclerotic dyslipidemia.

      Keywords

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