Posters 26 - 29 May, 2019 03. Lipids - 03.06 Cholesterol efflux and reverse cholesterol transport| Volume 287, e223, August 2019

Quality Control Of The Cholesterol Efflux Capacity Assay In A High Throughput Setting

      Background and Aims: The cholesterol efflux capacity (CEC) assay measures the functionality of HDL to induce efflux from macrophages in vitro. CEC inversely correlates with cardiovascular outcomes in most cohorts. To further elucidate the role of cholesterol efflux, additional large studies are needed. For this, a high-throughput capable protocol with stringent quality control is important to minimize technical variability. However, the BODIPY-cholesterol-based protocols used in the literature differ and, importantly, cell viability is not monitored on a per-well basis. Thus, the objective is to compare protocols and to introduce a per-well cell viability monitoring into the CEC assay.
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