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Cholesteryl ester metabolism in liver and blood plasma of various animal species

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      Summary

      In eight different animal species the activities of the following enzymes were determined: (1) the plasma lecithin:cholesterol acyltransferase (LACT); (2) the liver microsomal acyl-CoA:cholesterol acyltransferase; and (3) the liver lysosomal acid cholesterol esterase. In addition, the plasma and liver concentrations of free and esterified cholesterol were determined.
      The activities of the three enzymes differed considerably in the various animal species. The highest LCAT activity was observed in monkey and man, whereas calf displayed a rather low activity. In human no acyl-CoA:cholesterol acyltransferase could be demonstrated, and in liver from guinea pig a very low activity was observed. This is in contrast to the high activity found in rat liver. The highest cholesteryl ester hydrolyzing activity (at pH 4.5) was observed in rabbit and calf liver, whereas the hydrolytic activity in liver from rat, guinea pig, dog and swine was rather low.

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