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Intérêt et signification du test d'hyperlipémie par voie veineuse dans l'exploration des hyperlipémies étude de ses variations sous l'effet de l'alcool

  • J. Jaillard
    Affiliations
    Laboratoire de la Clinique Médicale Ouest, Cité Hospitalière, Place de Verdun, Lille 59000 (France)

    Laboratoire de Biochimie, Faculté de Médecine, Cité Hospitalière, Place de Verdun, Lille 59000 (France)

    Laboratoire de Physiopathologie des Lipides, Institut Pasteur, 20 Boulevard Louis XIV, 59012 Lille Cedex (France)
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  • P. Dewailly
    Affiliations
    Laboratoire de la Clinique Médicale Ouest, Cité Hospitalière, Place de Verdun, Lille 59000 (France)

    Laboratoire de Biochimie, Faculté de Médecine, Cité Hospitalière, Place de Verdun, Lille 59000 (France)

    Laboratoire de Physiopathologie des Lipides, Institut Pasteur, 20 Boulevard Louis XIV, 59012 Lille Cedex (France)
    Search for articles by this author
  • G. Sezille
    Correspondence
    Tirés à part: Dr. G. Sezille, Laboratoire de la Clinique Médicale Ouest, Cité Hospitalière, Place de Verdun, 59033 Lille Cedex (France).
    Affiliations
    Laboratoire de la Clinique Médicale Ouest, Cité Hospitalière, Place de Verdun, Lille 59000 (France)

    Laboratoire de Biochimie, Faculté de Médecine, Cité Hospitalière, Place de Verdun, Lille 59000 (France)

    Laboratoire de Physiopathologie des Lipides, Institut Pasteur, 20 Boulevard Louis XIV, 59012 Lille Cedex (France)
    Search for articles by this author
  • J.C. Fruchart
    Affiliations
    Laboratoire de la Clinique Médicale Ouest, Cité Hospitalière, Place de Verdun, Lille 59000 (France)

    Laboratoire de Biochimie, Faculté de Médecine, Cité Hospitalière, Place de Verdun, Lille 59000 (France)

    Laboratoire de Physiopathologie des Lipides, Institut Pasteur, 20 Boulevard Louis XIV, 59012 Lille Cedex (France)
    Search for articles by this author
  • C. Rault
    Affiliations
    Laboratoire de la Clinique Médicale Ouest, Cité Hospitalière, Place de Verdun, Lille 59000 (France)

    Laboratoire de Biochimie, Faculté de Médecine, Cité Hospitalière, Place de Verdun, Lille 59000 (France)

    Laboratoire de Physiopathologie des Lipides, Institut Pasteur, 20 Boulevard Louis XIV, 59012 Lille Cedex (France)
    Search for articles by this author
  • P. Scherpereel
    Affiliations
    Laboratoire de la Clinique Médicale Ouest, Cité Hospitalière, Place de Verdun, Lille 59000 (France)

    Laboratoire de Biochimie, Faculté de Médecine, Cité Hospitalière, Place de Verdun, Lille 59000 (France)

    Laboratoire de Physiopathologie des Lipides, Institut Pasteur, 20 Boulevard Louis XIV, 59012 Lille Cedex (France)
    Search for articles by this author
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      Summary

      The fractional removal rate of fat emulsion injected intravenously has been measured in several groups of subjects: controls, patients with hypercholesterolaemia or hypertriglyceridaemia. The intravenous fat tolerance test was also estimated in controls before and after a short ethanol perfusion.
      The fractional removal rate (K2) is low in patients with hypertriglyceridaemia and the results obtained suggest that the intravenous fat tolerance test provides a simple mean for an estimation of the removal of triglycerides from the blood. After a constant infusion of alcohol in healthy subjects a significant reduction of K2 is found.

      Résumé

      La mesure de la clearance fractionelle d'une émulsion lipidique injectée à faible dose a été effectuée chez plusieurs catégories de sujets normaux, athéroscléreux, hypercholestérolémiques et hypertriglycéridémiques. La mesure a par ailleurs été effectuée, chez des sujets sains, avant et après perfusion intraveineuse d'alcool.
      Cette clearance est diminuée chez les sujets hypertriglycéridémiques et les résultats obtenus permettent de penser qu'elle est représentative des capacités d'épuration lipidique plasmatique par les tissus extra-hépatiques. L'alcool induit, d'autre part, chez le sujet normal, un abaissement très significatif de la clearance fractionnelle.
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      References

        • Brewer Jr., H.B.
        • Lux S.E.
        • Ronan R.
        • John K.M.
        Amino acid sequence of human apoLp-Gln-II (apoA-II), an apolipoprotein isolated from the high-density lipoprotein complex.
        Proc. Nat. Acad. Sci. U.S. 1972; 69: 1304
        • Shore B.
        • Shore V.
        Some physical and chemical studies on the protein moiety of a highdensity (1.12–1.195g/ml) lipoprotein fraction of human serum.
        Biochemistry. 1967; 6: 1962
        • Gustafson A.
        • Alaupović P.
        • Furman R.H.
        Studies of the composition and structure of serum lipoproteins: physical-chemical characterization of phospholipid-protein residues obtained from very-low-density human serum lipoproteis.
        Biochim. Biophys. Acta. 1964; 84: 767
        • Gustafson A.
        • Alaupović P.
        • Furman R.H.
        Studies of the composition and structure of serum lipoproteins. Separation and characterization of phospholipid-protein residues obtained by partial delipidization of very low density lipoproteins of human serum.
        Biochemistry. 1966; 5: 632
        • Ledford J.H.
        • Alaupović P.
        • Furman R.H.
        Studies on the protein moieties (apolipoproteins) of very low density lipoproteins of hyperlipemic human serum.
        Circulation. 1966; 34 (Abs.): 18
        • Alaupović P.
        Recent advances in metabolism of plasma lipoproteins: Chemical aspects.
        Progr. Biochem. Pharmacol. 1968; 4: 91
        • Lee D.M.
        • Alaupović P.
        Studies of the composition and structure of plasma lipoproteins. Isolation, composition, and immunochemical characterization of low density lipoprotein subfractions of human plasma.
        Biochemistry. 1970; 9: 2244
        • Alaupović P.
        • Lee D.M.
        • McConathy W.J.
        Studies on the composition and structure of plasma lipoproteins: distribution of lipoprotein families in major density classes of normal human plasma lipoproteins.
        Biochim. Biophys. Acta. 1972; 260: 689
        • Nichols A.V.
        Human serum lipoproteins and their interrelationships.
        Advan. Biol. Med. Phys. 1967; 11: 109
        • Alaupović P.
        • Furman R.H.
        • Falor W.H.
        • Sullivan M.L.
        • Walraven S.L.
        • Olson A.C.
        Isolation and characterization of human chyle chylomicrons and lipoproteins.
        Ann. N.Y. Acad. Sci. 1968; 149: 791
        • Alaupović P.
        Apolipoproteins and lipoproteins.
        Atherosclerosis. 1971; 13: 141
        • Shore B.
        • Shore V.
        Isolation and characterization of polypeptides of human serum lipoproteins.
        Biochemistry. 1969; 8: 4510
        • Brown W.V.
        • Levy R.I.
        • Fredrickson D.S.
        Studies of the proteins in human plasma very low density lipoproteins.
        J. Biol. Chem. 1969; 244: 5687
        • Kane J.P.
        • Richard E.G.
        • Havel R.J.
        Subunit heterogeneity in human serum beta lipoprotein.
        Proc. Nat. Acad. Sci. U.S. 1970; 66: 1075
        • Shore V.
        • Shore B.
        Heterogeneity of human plasma very low density lipoproteins. Separation of species differing in protein components.
        Biochemistry. 1973; 12: 502
        • Fredrickson D.S.
        • Levy R.I.
        • Lees R.S.
        Fat transport in lipoproteins — An integrated approach to mechanisms and disorders.
        N. Engl. J. Med. 1967; 267: 273
        • Lees R.S.
        Immunoassay of plasma low-density lipoproteins.
        Science. 1970; 169: 493
        • DeLalla O.
        • Gofman J.W.
        Ultracentrifugal analysis of serum lipoproteins.
        in: Glick D. Methods of Biochemical Analysis. Vol. 1. Wiley-Interscience, New York1954: 459
        • Havel R.J.
        • Eder H.A.
        • Bragdon J.H.
        The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum.
        J. Clin. Invest. 1955; 34: 1345
        • Scanu A.M.
        • Edelstein C.
        Solubility in aqueous solutions of ethanol of the small molecular weight peptides of the serum very low density and high density lipoproteins: Relevance to the recovery problem during delipidation of serum lipoproteins.
        Anal. Biochem. 1971; 44: 576
        • Gotto A.M.
        • Levy R.I.
        • Rosenthal A.S.
        • Fredrickson D.S.
        Human serum betalipoprotein.
        Nature (London). 1968; 219: 1157
        • Davis B.J.
        Disc electrophoresis, Part 2 (Method and application to human serum proteins).
        Ann. N. Y. Acad. Sci. 1964; 121: 404
        • Ouchterlony Ö.
        Antigen-antibody reactions in gels, Part 6 (Types of reactions in coordinated systems of diffusion).
        Acta Pathol. Microbiol. Scand. 1953; 32: 231
        • Grabar P.
        • Williams Jr., C.A.
        Méthode immunoélectrophorétique d'analyse mélanges de substances antigéniques.
        Biochim. Biophys. Acta. 1955; 17: 67
        • Liu T.Y.
        • Chang Y.H.
        Hydrolysis of proteins with p-toluenesulfonic acid. Determination of tryptophan.
        J. Biol. Chem. 1971; 246: 2842
        • Moore S.
        On the determination of cystine as cysteic acid.
        J. Biol. Chem. 1963; 238: 235
        • Lowry O.H.
        • Rosebrough N.J.
        • Farr A.L.
        • Randall R.J.
        Protein measurement with the Folin phenol reagent.
        J. Biol. Chem. 1951; 193: 265
        • Gustafson A.
        • Alaupović P.
        • Furman R.H.
        Studies of the composition and structure of serum lipoproteins: Isolation, purification, and characterization of very low density lipoproteins of human serum.
        Biochemistry. 1965; 4: 596
        • Sperry W.M.
        • Webb A.
        A revision of the Schoenheimer-Sperry method for cholesterol determination.
        J. Biol. Chem. 1950; 187: 97
        • Van Handel E.
        • Zilversmit D.B.
        Micromethod for the direct determination of serum triglycerides.
        J. Lab. Clin. Med. 1957; 50: 152
        • Gerlach E.
        • Deuticke B.
        Eine einfache Methode zur Mikrobestimmung von Phosphat in der Papierchromatographie.
        Biochem. Z. 1963; 337: 477
        • Levy R.I.
        • Lees R.S.
        • Fredrickson D.S.
        The nature of pre-beta (very low density) lipoproteins.
        J. Clin. Invest. 1966; 45: 63
        • Kook A.I.
        • Eckhaus A.S.
        • Rubinstein D.
        The dissociation in vitro of the α- and β-lipoprotein components of human and rat very low density lipoproteins.
        Can. J. Biochem. 1970; 48: 712
        • Pearlstein E.
        • Eggena P.
        • Aladjem F.
        The human serum high density lipoprotein peptides of very low density lipoproteins and chylomicrons — An appendix.
        Immunochemistry. 1971; 8: 865
        • Albers J.J.
        • Chen C.H.
        • Aladjem F.
        Human serum lipoproteins. Evidence for three classes of lipoproteins in Sf 0–2.
        Biochemistry. 1972; 11: 57
        • Albers J.J.
        • Scanu A.M.
        Isoelectric fractionation and characterization of polypeptides from human serum very low density lipoproteins.
        Biochim. Biophys. Acta. 1971; 236: 29
        • Levy R.I.
        • Fredrickson D.S.
        Heterogeneity of plasma high density lipoproteins.
        J. Clin. Invest. 1965; 44: 426
        • Margolis S.
        • Langdon R.G.
        Studies on human serum β1-lipoprotein, Part I (Amino acid composition).
        J. Biol. Chem. 1966; 241: 469
        • Granda J.L.
        • Scanu A.
        Solubilization and properties of the apolipoproteins of the very low- and low-density lipoproteins of human serum.
        Biochemistry. 1966; 5: 3301
        • Smith R.
        • Dawson J.R.
        • Tanford C.
        The size and number of polypeptide chains in human serum low density lipoprotein.
        J. Biol. Chem. 1972; 247: 3376
        • Gotto A.M.
        • Brown W.V.
        • Levy R.I.
        • Birnbaumer M.E.
        • Fredrickson D.S.
        Evidence for the identity of the major apoprotein in low density and very low density lipoproteins in normal subjects and patients with familial hyperlipoproteinemia.
        J. Clin. Invest. 1972; 51: 1486
        • Shore V.G.
        • Shore B.
        The apolipoproteins. Their structure and functional roles in humanserum lipoproteins.
        in: Nelson G.J. Blood Lipids and Lipoproteins: Quantitation, Composition, and Metabolism. Wiley-Interscience, New York1972: 789
        • Shelburne F.A.
        • Quarfordt S.H.
        A new apolipoprotein of human plasma very low density lipoprotein.
        Fed. Proc. 1973; 32 (Abs.): 547
        • Marshall W.E.
        • Kummerow F.
        The carbohydrate constituents of human serum β-lipoprotein: galactose, mannose, glucosamine and sialic acid.
        Arch. Biochem. Biophys. 1962; 98: 271
        • Kane J.P.
        A rapid electrophoretic technique for identification of subunit species of apoproteins in serum lipoproteins.
        Anal. Biochem. 1973; 53: 350
        • Kostner G.
        Isolation of lipoprotein A with hydrated density characteristic for low density lipoproteins.
        FEBS Lett. 1972; 20: 25
        • Puppione D.L.
        • Forte G.M.
        • Nichols A.V.
        • Strisower E.H.
        Partial characterization of serum lipoproteins in the density interval 1.04–1.06 g/ml.
        Biochim. Biophys. Acta. 1970; 202: 392
        • Alcindor L.G.
        • Infante R.
        • Soler-Argilaga C.
        • Raisonnier A.
        • Polonovski J.
        • Caroli J.
        Induction of the hepatic synthesis of β-lipoproteins by high concentrations of fatty acids. Effect of actinomycin D.
        Biochim. Biophys. Acta. 1970; 210: 483
        • Lee D.M.
        • Alaupović P.
        On the existence of lipoprotein families both as associations and as separate entities in the low density lipoproteins of normal human plasma.
        Circulation. 1972; 46 (Abs.): 268
        • Pearlstein E.
        • Aladjem F.
        Subpopulations of human serum very low density lipoproteins.
        Biochemistry. 1972; 11: 2553