Mononuclear cell adhesion to collagen ex vivo is related to pulse pressure in elderly subjects


      Mononuclear cells and platelets are intimately involved in the pathogenesis and complications of cardiovascular disease. Platelet activation has been reported in hypertension, though the activation-state of monocytes has received less attention. In this study the adhesiveness of monocytes and platelets was assessed and any relationship between the adhesive properties of these cellular elements and plasma levels of soluble adhesion molecules and blood pressure parameters determined. Fifty six elderly volunteers, of whom 32 were classified hypertensive (daytime SBP≥135 mmHg), underwent 24 h blood pressure monitoring, assessment of monocyte and platelet adhesion and measurement of the plasma soluble adhesion molecules ICAM-1, L-selectin, E-selectin and vWF. In the elderly hypertensive subjects, monocyte adhesion to collagen coated (P<0.05) and tissue culture plastic microwells (P<0.05) was significantly elevated and circulating levels of soluble ICAM-1 (P<0.01) and soluble E-selectin (P<0.05) were significantly raised compared to their normotensive counterparts. A significant correlation was found to exist between monocyte adhesion to collagen and daytime pulse pressure (r=0.39, P<0.01) and also between plasma levels of soluble E-selectin and clinic DBP (r=0.40, P<0.001). The increased monocyte adhesion witnessed in hypertensive subjects and with increasing pulse pressure may contribute to the increased risk of cardiovascular disease in hypertension. Whether this increased adhesiveness is a property of the monocytes, or reflects endothelial cell activation, remains to be determined.


      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'


      Subscribe to Atherosclerosis
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect


        • Ross R.
        The pathogenesis of atherosclerosis: a pathogenesis for the 1990s.
        Nature. 1993; 362: 801
        • Andrioli G.
        • Ortolani R.
        • Fontana L.
        • Gaino S.
        • Bellavite P.
        • Lechi C.
        • Minuz P.
        • Manzato F.
        • Tridente G.
        • Lechi A.
        Study of platelet adhesion in patients with uncomplicated hypertension.
        J. Hypertens. 1996; 14: 1215
        • Valtier D.
        • Guicheney P.
        • Bandouin-Legros M.
        • Meyer P.
        Platelets in human essential hypertension: in vitro hyperreactivity to thrombin.
        J. Hypertens. 1986; 4: 551
        • Ashida T.
        • Tanaka T.
        • Yokouchi M.
        • Kuramochi M.
        • Deguchi F.
        • Kimura G.
        • Kojima S.
        • Ito K.
        • Ikeda M.
        Effect of dietary sodium on platelet alpha-2 adrenergic receptors in essential hypertension.
        Hypertension. 1985; 7: 972
        • Mehta J.
        • Mehta P.
        Platelet function in hypertension and effect of therapy.
        Am. J. Cardiol. 1981; 47: 331
        • Coccheri S.
        • Fiorentine P.
        Platelet adhesiveness and aggregation in hypertensive patients.
        Acta Med. Scand. (Suppl). 1971; 525: 273
        • Gerrity R.G.
        The role of monocyte in atherogenesis. I. Transition of blood borne monocytes into foam cells in fatty lesions.
        Am. J. Pathol. 1981; 103: 181
        • Simon B.C.
        • Haudenschild C.C.
        • Cohen R.A.
        • Palacino J.
        Preservation of endothelium-dependent relaxation in atherosclertic rabbit aorta by probucol.
        J. Cardiovasc. Pharmacol. 1993; 21: 893
        • Lockette W.
        • Otsuka Y.
        • Carretero O.
        The loss of endothelium dependent vascular relaxation in hypertension.
        Hypertension. 1986; 8: II161
        • Ball K.
        • Turner R.
        Smoking and the heart. The basis for action.
        Lancet. 1974; 2: 822
        • Butcher E.C.
        Leucocyte-endothelial cell recognition: three (or more) steps to specificity and diversity.
        Cell. 1991; 67: 1033
        • Takahashi M.
        • Ikeda U.
        • Masuyama J.-I.
        • Kitagawa S.-I.
        • Kasahara T.
        • Saito M.
        • Kano S.
        • Shimada K.
        Involvement of adhesion molecules in human monocyte adhesion to and transmigration through endothelial cells in vitro.
        Atherosclerosis. 1994; 108: 73
        • Bøyum A.
        Isolation of mononuclear cells and granulocytes from human blood.
        J. Clin. Invest. 1968; 21: 77
        • Bath P.M.W.
        • Booth R.F.G.
        • Hassall D.G.
        Monocyte–lymphocyte discrimination in a new microtitre-based adhesion assay.
        J. Immunol. Methods. 1989; 118: 59-65
        • Bellavite P.
        • Andrioli G.
        • Guzzo P.
        • Arigliano P.
        • Chirumbolo S.
        • Manzato F.
        • Santonastaso C.
        A colorimetric method for the measurement of platelet adhesion in microtitre plates.
        Anal. Biochem. 1994; 216: 444
        • Mancia G.
        • Sega R.
        • Bravi C.
        • De Vito G.
        • Valagussa F.
        • Cesana G.
        • Zanchetti A.
        Ambulatory blood pressure normality: results from the PAMELA study.
        J. Hypertens. 1995; 13: 1377
        • Manning G.
        • Rushton L.
        • Millar-Craig M.W.
        Twenty four hour ambulatory blood pressure: a sample from a normal British population.
        J. Hum. Hypertens. 1998; 12: 123
        • McCarron R.M.
        • Wang L.
        • Sirén A.-L.
        • Spatz M.
        • Hallenbeck J.M.
        Monocyte adhesion to cerebromicrovascular endothelial cells derived from hypertensive and normotensive rats.
        Am. J. Physiol. 1994; 267: H2491
        • Kim J.A.
        • Berliner J.A.
        • Nadler J.L.
        Angiotensin II increases monocyte binding to endothelial cells.
        Biochem. Biophys. Res. Commun. 1996; 226: 862
        • Liu Y.
        • Liu T.
        • McCarron R.M.
        • Spatz M.
        • Feuerstein G.
        • Hallenbeck J.M.
        • Sirén A.-L.
        Evidence for activation of endothelium and monocytes in hypertensive rats.
        Am. J. Physiol. 1996; 270: H2125
        • Tropea B.I.
        • Huie P.
        • Cooke J.P.
        • Tsao P.S.
        • Sibley R.K.
        • Zarins C.K.
        Hypertension-enhanced monocyte adhesion in experimental atherosclerosis.
        J. Vasc. Surg. 1996; 23: 596
        • McCarron R.M.
        • Wang L.
        • Sirén A.-L.
        • Spatz M.
        • Hallenbeck J.M.
        Adhesion molecules on normotensive and hypertensive rat brain endothelial cells.
        Proc. Soc. Exp. Biol. Med. 1994; 205: 257
        • Caper I.V.Q.
        • Alexander R.W.
        • Lou P.
        • De Leon H.
        • Wilcox J.N.
        • Ishizaka N.
        • Howard A.B.
        • Taylor W.R.
        Monocyte chemoattractant protein-1 expression in aortic tissue of hypertensive rats.
        Hypertension. 1997; 30: 1397
        • Wang D.L.
        • Wung B.-S.
        • Shyy Y.-J.
        • Lin C.-F.
        • Chao Y.-J.
        • Usami S.
        • Chien S.
        Mechanical strain induces monocyte chemotactic protein-1 gene expression in endothelial cells: effects of mechanical strain on monocyte adhesion to endothelial cells.
        Circ. Res. 1995; 77: 294
        • Madhavan S.
        • Ooi W.
        • Cohen H.
        • Alderman M.H.
        Relation of pulse pressure and blood pressure reduction to the incidence of myocardial infarction.
        Hypertension. 1994; 23: 395
        • Flack J.M.
        • Neaton J.
        • Grimm R.
        • et al.
        Blood pressure and mortality among men with prior myocardial infarction.
        Circulation. 1995; 92: 2437
        • Bots M.L.
        • Witteman J.C.M.
        • Hofman A.
        • de Jong P.T.V.M.
        • Grobbee D.E.
        Low diastolic blood pressure and atherosclerosis in elderly subjects.
        Arch. Int. Med. 1996; 156: 843
        • Bath P.M.
        • Gladwin A.M.
        • Martin J.F.
        Human monocyte characteristics are altered in hypercholesterolaemia.
        Atherosclerosis. 1991; 90: 175
        • Lösche W.
        • Krause S.
        • Pohl A.
        • Pohl C.
        • Liebrenz A.
        • Schauer I.
        • Rühling K.
        • Till U.
        Functional behaviour of mononuclear blood cells from patients with hypercholesterolemia.
        Thromb. Res. 1992; 65: 337
        • Stragliotto E.
        • Camera M.
        • Postiglione A.
        • Sirtori M.
        • Di Minno G.
        • Tremoli E.
        Functionally abnormal monocytes in hypercholesterolemia.
        Arterioscler. Thromb. 1993; 13: 944
        • Gearing A.J.H.
        • Hemingway I.
        • Pigott R.
        • Hughes J.
        • Rees A.J.
        • Cashman S.J.
        Soluble forms of vascular adhesion molecules, E-selectin, ICAM-1 and VCAM-1: pathological significance.
        Ann. N. Y. Acad. Sci. 1992; 667: 324
        • Blann A.D.
        • Wai T.
        • Maxwell S.J.R.
        • Waite M.A.
        Increased levels of the soluble adhesion molecule E-selectin in essential hypertension.
        J. Hypertens. 1994; 12: 925
        • Adams D.H.
        • Mainolfi E.
        • Elias E.
        • Neuberger J.M.
        • Rothlein R.
        Detection of circulating intercellular adhesion molecule-1 after liver transplantation, evidence of local release within the liver during graft rejection.
        Transplantation. 1993; 55: 83
        • Ballantyne C.M.
        • Mainolfi E.A.
        • Young J.B.
        • Windsor N.T.
        • Cocanougher B.
        • Lawrence E.C.
        • Pollack M.S.
        • Entman M.L.
        • Rothlein R.
        Relationships of increased levels of circulating intercellular adhesion molecule-1 after heart transplantation to rejection: human leukocyte antigen mismatch and survival.
        J. Heart Lung. Trans. 1994; 13: 597
        • Schleiffenbaum B.
        • Olivier S.
        • Tedder T.F.
        Soluble L-selectin is present in human plasma at high levels and retains functional activity.
        J. Cell. Biol. 1992; 119: 229
        • Lo S.K.
        • Lee S.
        • Ramos R.A.
        • Lobb R.
        • Rosa M.
        • Chi-Rosso G.
        • Wright S.D.
        Endothelial-leukocyte adhesion molecule-1 stimulates the adhesive activity of leukocyte integrin CR3 (CD11b/CD18, Mac-1,α1β2) on human neutrophils.
        J. Exp. Med. 1991; 173: 1493