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Research Article| Volume 3, ISSUE 1, P24-31, January 02, 1963

On the alleged interaction in vitro between plasma β-lipoproteins and aortic mucopolysaccharides

DOI:https://doi.org/10.1016/S0368-1319(63)80092-7
On the alleged interaction in vitro between plasma β-lipoproteins and aortic mucopolysaccharides
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      Summary

      A recent report that complex formation between arterial wall mucopolysaccharides (MPS) and plasma β-lipoproteins can be observed by paper electrophoresis has been re-investigated under a wide variety of electrophoretic conditions and with various aortic MPS and fractions thereof. No complex formation could be detected. Results of other authors may possibly be attributed to the use of MPS preparations containing attached protein residues. While interaction between bound arterial MPS and plasma proteins in vivo is not necessarily excluded, it is believed that interaction between pure isolated MPS and intact plasma proteins does not occur during paper electrophoresis.

      Résumé

      L'objet d'une communication récente, selon laquelle une formation complexe peut être observée entre les mucopolysaccharides (MPS) de la paroi artérielle et les lipoprotéines-β plasmatiques, a été étudié de nouveau sous différentes conditions électrophorétiques et avec diverses fractions de MPS aortiques. On ne put trouver de formation complexe. Les constatations d'autres chercheurs pourraient être attribuées à l'utilisation de préparations contenant des résidues protéiques attachés. Tandis que l'interréaction in vivo entre les MPS artériels et les protéines plasmatiques n'est pas nécessairement exclue, on croit qu'il n'y a pas d'interréaction pendant l'électrophorèse sur papier entre les MPS isolés purs et les protéines plasmatiques intactes.

      Zusammenfassung

      Ein unlängst erschienener Bericht, dass sich die Komplexbildung zwischen Mukopolysacchariden (MPS) der Aortawand und β-Lipoproteinen im Plasma mittels Papierelektrophorese wahrnehmen lasse, wurde erneut untersucht, und zwar unter mannigfaltiger Variation der elektrophoretischen Bedingungen und mit unterschiedlichen MPS und MPS-Fraktionen der Aorta. Komplexbildung wurde nicht beobachtet. Die Resultate anderer Untersucher beruhen wohl auf dem Umstand, dass MPS-Präparationen zur Anwendung kamen die Proteinreste enthielten. Obschon sich Wechselwirkungen zwischen gebundenen arteriellen MPS und Plasmaproteinen in vivo nicht notwendigerweise ausschliessen lassen, darf man doch vermuten, dass sie zwischen rein isolierten MPS und intakten Plasmaproteinen während Papierelektrophorese nicht vorkommen.
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      Article info

      Publication history

      Received: September 13, 1962

      Identification

      DOI: https://doi.org/10.1016/S0368-1319(63)80092-7

      Copyright

      © 1963 Published by Elsevier Inc.

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